电离辐射通过转化生长因子-β-介导的上皮-间质转换来促进癌细胞的摧残迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

摘录 ：探讨远红外支线是否可通过转化激酶-β（TGF-β）-介导的内膜-间质变换 (EMT)来促进抗体的蹂躏迁移。应用于工业产值2Gy(60)Coγ支线紫外光起源于人类心脏的6种抗体，据信与EMT具体的变化，这包括分别利用光学仪器新技术，受体质印迹方法，免疫荧光新技术，划痕飞行测试和TranswellWHITE飞行测试来观察并检测细胞核组织形态，EMT标记，蹂躏迁移能力等。采用酶联免疫吸附法检测这些抗体中TGF-β受体高度，利用特别衍生物SB431542来评核TGF-β频率渠道在远红外支线EMT中的作用。经过工业产值为2Gy紫外光的抗体中普遍存在间叶细胞核的表达，与骗紫外光组相对其内膜标记减小，间叶细胞核标记增加，同时其蹂躏转移能力减慢，TGF-β受体高度也提高。全面发掘出由A549远红外支线可借的EMT可通过对TGF-β频率抑制作用遭遇再一。这些得出结论TGF-β介导的EMT在远红外支线可借减慢抗体蹂躏转移能力中起着关键作用。

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