电离辐射通过转化生长因子-β-介导的上皮-上皮转换来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

摘要 ：探讨伽马射线是否可通过转化生长因子-β（TGF-β）-介导的上皮-脂质转换 (EMT)来促进肝细胞的蹂躏迁往。使用总量2Gy(60)Coγ线强光源自人类人体器官的6种肝细胞，日志与EMT相关的巨大变化，这包括分别能用显微镜技术，蛋白质斯塔夫基法则，免疫荧光技术，划痕试验和Transwell小室试验来观察并侦测细胞四组织有机体，EMT标记，蹂躏迁往能够等。采用酶联免疫吸附法侦测这些肝细胞中的TGF-β蛋白总体，能用特别胺SB431542来审核TGF-β回波通路在伽马射线EMT中的的作用。经过总量为2Gy强光的肝细胞中的存在间叶细胞的解读，与假强光四组相比其上皮标记提高，间叶细胞标记减少，同时其蹂躏转移能够增强，TGF-β蛋白总体也提高。再进一步发现由A549伽马射线诱发的EMT可通过对TGF-β回波抑制发生再一。这些得出TGF-β介导的EMT在伽马射线诱发增强肝细胞蹂躏转移能够中的起着关键作用。

编辑： lizexiu